Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy

Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy

April 06, 2019 0 Comments

Two-Photon Probes for Golgi Apparatus: Detection of Golgi Apparatus in Live Tissue by Two-Photon Microscopy - Analytical Chemistry (ACS Publications) ADVERTISEMENT Log In Register Cart ACS ACS Publications C&EN CAS ACS Journals ACS eBooks C&EN Global Enterprise A Accounts of Chemical Research ACS Applied Bio Materials ACS Applied Electronic Materials - New in 2019 ACS Applied Energy Materials ACS Applied Materials & Interfaces ACS Applied Nano Materials ACS Applied Polymer Materials - New in 2019 ACS Biomaterials Science & Engineering ACS Catalysis ACS Central Science ACS Chemical Biology ACS Chemical Neuroscience ACS Combinatorial Science - Journal of Combinatorial Chemistry ACS Earth and Space Chemistry ACS Energy Letters ACS Infectious Diseases ACS Macro Letters ACS Materials Letters - New in 2019 ACS Medicinal Chemistry Letters ACS Nano ACS Omega ACS Pharmacology & Translational Science ACS Photonics ACS Sensors ACS Sustainable Chemistry & Engineering ACS Synthetic Biology Analytical Chemistry - I&EC Analytical Edition B Biochemistry Bioconjugate Chemistry Biomacromolecules Biotechnology Progress C Chemical Research in Toxicology Chemical Reviews Chemistry of Materials Crystal Growth & Design E Energy & Fuels Environmental Science & Technology Environmental Science & Technology Letters I Industrial & Engineering Chemistry - Journal of Industrial & Engineering Chemistry Industrial & Engineering Chemistry Research - I&EC Process Design and Development - I&EC Fundamentals - Product Research & Development - Product R&D - I&EC Product Research and Development Inorganic Chemistry J Journal of the American Chemical Society Journal of Agricultural and Food Chemistry Journal of Chemical & Engineering Data - I&EC Chemical & Engineering Data Series Journal of Chemical Education Journal of Chemical Information and Modeling - Journal of Chemical Documentation - Journal of Chemical Information and Computer Sciences Journal of Chemical Theory and Computation Journal of Medicinal Chemistry Journal of Natural Products The Journal of Organic Chemistry J (continued) The Journal of Physical Chemistry A The Journal of Physical Chemistry B The Journal of Physical Chemistry C - The Journal of Physical Chemistry - The Journal of Physical and Colloid Chemistry The Journal of Physical Chemistry Letters Journal of Proteome Research L Langmuir M Macromolecules Molecular Pharmaceutics N Nano Letters O Organic Letters Organic Process Research & Development Organometallics About ACS eBooks ACS Reagent Chemicals ACS Style Guide Advances in Chemistry ACS Symposium Series Advanced Search Search Citation Subject Search in:Anal. 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Chem., Article ASAP DOI: 10.1021/acs.analchem.9b00607 Publication Date (Web): March 28, 2019 Copyright © 2019 American Chemical Society *E-mail: [email protected] Cite this: Anal. Chem.  XXXX ,  XXX ,  XXX-XXX Abstract We have developed blue- and yellow-emitting two-photon probes (BGolgi-blue and PGolgi-yellow) from 6-(benzo[d]oxazol-2-yl)-2-naphthalylamine and 2,5-bis(benzo[d]oxazol-2-yl)pyrazine derivatives as the fluorophores and trans-Golgi-network peptide (SDYQRL) as the Golgi-apparatus-targeting moiety. HeLa cells labeled with BGolgi-blue and PGolgi-yellow emitted two-photon-excited fluorescence at 462 and 560 nm, respectively, with effective two-photon-action cross-section values of 1860 and 1600 × 10–50 cm4·s/photon, respectively. The probes can detect the Golgi apparatus in live cells and deep inside live tissue via two-photon microscopy at widely separated wavelength regions with high selectivity and minimal pH interference, and they are photostable and have low cytotoxicity. The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.analchem.9b00607. Experimental details on the synthesis of compounds, spectroscopic characterization of compounds, viability of HeLa cells in the presence of BGolgi-blue and PGolgi-yellow probes, TPM images of HeLa cells labeled with BGolgi-blue and PGolgi-yellow, effect of the pH on the one-photon-fluorescence intensity of BGolgi-blue and PGolgi-yellow, TPM images of a brain tissue colabeled with PGolgi-yellow and ABI-Nu, 1H and 13C NMR spectra, and HRMS spectra (PDF) View: ACS ActiveView PDF | PDF | PDF w/ Links | Full Text HTML PDF ac9b00607_si_001.pdf(2.32 MB) Related Content Related Content: Discovery of Turn-On Fluorescent Probes for Detecting Bcl-2 Protein Analytical Chemistry Liu, Dong, Xu, Han, Fang, Huang, Zhou, Du, and Li0 (0), Abstract: B-cell-lymphoma-2-gene (Bcl-2) family proteins play a central role in regulating programmed cell death. In cancer, antiapoptotic Bcl-2 proteins, such as Bcl-2 and Mcl-1, are overexpressed. However, there are few developed labeling techniques for tracing ... Abstract | Full Text HTML | PDF w/ Links | Hi-Res PDF Evaluation of Glutathione S-Transferase Inhibition Effects on Idiopathic Pulmonary Fibrosis Therapy with a Near-Infrared Fluorescent Probe in Cell and Mice Models Analytical Chemistry He, Bai, Huang, Xing, Chen, Yu, and Lv0 (0), Abstract: Idiopathic pulmonary fibrosis (IPF) is a lung-limited and progressive fibrotic disease. The early diagnosis and therapies of IPF are still full of clinical challenges. Glutathione S-transferase (GSTs) plays significant roles in promoting the formation of ... Abstract | Full Text HTML | PDF w/ Links | Hi-Res PDF De Novo Design of Chemical Stability Near-Infrared Molecular Probes for High-Fidelity Hepatotoxicity Evaluation In Vivo Journal of the American Chemical Society Cheng, Peng, Lv, Su, Liu, Chen, Yuan, and Zhang0 (0), Abstract: Near-infrared (NIR) fluorescence imaging technique is garnering increasing research attention due to various advantages. However, most NIR fluorescent probes still suffer from a false signals problem owing to their instability in real application. ... Abstract | Full Text HTML | PDF w/ Links | Hi-Res PDF Peroxynitrite Activatable NIR-II Fluorescent Molecular Probe for Drug-Induced Hepatotoxicity Monitoring Analytical Chemistry Li, Wang, Lei, Sun, El-Toni, Alhoshan, Fan, and Zhang 2019 91 (7), pp 4771–4779 Abstract: Drug-induced hepatotoxicity represents an important challenge for safety in drug development. The production of peroxynitrite (ONOO–) is proposed as an early sign in the progression of drug-induced hepatotoxicity. Currently, reported ONOO– probes mainly ... Abstract | Full Text HTML | PDF w/ Links | Hi-Res PDF In Vivo Imaging of Endogenously Produced HClO in Zebrafish and Mice Using a Bright, Photostable Ratiometric Fluorescent Probe Analytical Chemistry Duan, Won, Verwilst, Xu, Kim, Zeng, and Kim 2019 91 (6), pp 4172–4178 Abstract: A high brightness red fluorescent probe (S-BODIPY) has been developed for the sensitive and specific imaging of HClO/ClO– in vitro and in vivo. This probe exhibits some distinctive features such as excellent resistance to photobleaching, a high ... Abstract | Full Text HTML | PDF w/ Links | Hi-Res PDF BODIPY-Based Fluorescent Probe for Dual-Channel Detection of Nitric Oxide and Glutathione: Visualization of Cross-Talk in Living Cells Analytical Chemistry Chen, Niu, Shao, and Yang 2019 91 (7), pp 4301–4306 Abstract: Nitric oxide (NO) and glutathione (GSH) have interplaying roles in oxidant-antioxidant balance. In this work, we developed the first example of a single fluorescent probe that displayed a turn-on fluorescence response toward NO and GSH from dual emission ... Abstract | Full Text HTML | PDF w/ Links | Hi-Res PDF



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